Nanoliter 2010 Instruction Manual

MICRO2T SMARTouch Controller Instruction Manual (for use with the UMP3 and Nanoliter2010)

FAQ for Nanoliter Injector with SMARTouch Controller

FAQ:

My Nanoliter with SMARTouch Controller has liquid leaking out or I can see bubbles forming inside the pipette. What can cause this and what action can I take to correct the problem?

Answer:

1) Easy to fix, as it is probably the seal rings. Check to make sure the seal rings are assembled in the correct sequence.

2) The plunger seal has a conical shaped hole in the center. The seal should be  installed with the larger end of the hole pushed onto the plunger first. This means the smaller end of the hole is facing toward the tip of the plunger. This is shown graphically in the lower diagram below. Pay special attention to the notes in red.  If the plunger seal slides off easily by gravity when the Nanoliter Injector is held vertically pointing downward, the seal hole is too large and it will leak. Replace the seal.

The plunger seal should not slide off the plunger shaft under gravity when  tested by holding the nanoliter injector vertically with the tip pointing downward (with no other seals installed). There should be some friction when pushing the plunger seal onto the plunger and it should grip the plunger slightly. It should not be loose.

3) The orientation of the plastic spacer and large glass seal (reddish color with large hole for capillary glass) must be oriented correctly as shown. The shank end of the capillary must be correctly seated into the recess in the plastic spacer.

4) The seals should and capillary glass should be clean. If the seals are oily, they are more likely to leak. Use isopropyl alcohol to clean the seals, and if necessary immerse them in it to get the oil out of the hole.

5) Tighten the cap securely.

*Ejection rate is limited by pipette tip opening size. Controller injection rate should not be set to exceed pipette capacity

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