Make micropipettes to record cellular concentrations
- 0.1 mL
WPI’s Liquid Ion Exchangers (LIX), for use with the FD223A Electrometer, allow intracellular measurements to be made for cations (hydrogen, potassium and calcium).
Liquid Ion Exchanger Instruction Manual
ION | H+ | K+ | Ca++ |
CATALOG NO. | IE010 | IE190 | IE200 |
SELECTIVITY COEFFICIENTS* | |||
Na+ | 12.7 | 1.97 | 5.5 |
Mg++ | — | 2.95 | 4.9 |
K+ | — | — | 5.4 |
Ca++ | — | 2.7 | — |
USEFUL pH RANGE | 2-10 | 4-10 | 4-10 |
SLOPE | 56 mV | 58 mV | 28 mV |
LINEAR RANGE | pH 4-12 | pK 0-3 | pCa 1-7 |
APPROX. EQUIV. | — | Corning477317 | ETH1001 |
*Selectivity Coefficients are expressed here as -log Kij or pKij. |
S. Ibusuki, H. Katsuki, M. Takasaki "The Effects of Extracellular pH with and without Bicrbonate on Intracellular Procaine Concentrations and Anesthetic Effects in Crayfish Giant Axons [Laborator Investigations]" Anesthesiology 88. 1998: 1549-1557