Description:

The murine monoclonal antibody to globo H (clone MBr1) is raised against the breast cancer cell line MCF7 and recognizes a saccharidic epitope (CaMBr1) overexpressed on a high percentage of human breast, ovary lung and prostate carcinomas. This antigen was originally identified on the immunogen as a globo-series glycosphingolipid with a H-like determinant at its terminus (globo-H), identical to GL6 isolated from human teratocarcinoma. MAb to MBr1 was also found to be reactive with different glycoconjugates (glycoproteins, mucins and glycolipids) in breast and small cell lung carcinoma. Retrospective as well as prospective studies suggest that CaMBr1 expression is associated with tumor aggressiveness and survival outcome in breast and small cell lung cancer and that poor prognosis is more frequently associated with CaMBr1 expression on glycoproteins. The epitope recognized by the MAb to MBr1 consists of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal, available as a synthetic oligosaccharide exploitable for the development of new anti-cancer vaccines. Carbohydrate antigens expressed at the cell surface of human cancer cells hold promise as targets for active immunization with vaccines.CaMBR1 is also expressed on some normal tissues, and its expression restricted tissue distribution makes it a suitable target for oncologic clinical applications. In particular the MAb to MBr1 can be succesfully used for immunocytochemical detection of micrometastatic cells.

Product Specification:

Clone:	MBr1
Host:	Mouse
Isotype:	IgM
Immunogen:	Membrane preparation from the human breast carcinoma cell line MCF7.
Species reactivity:	Human, RatCat, Dog
Specificity:	Recognizes membrane bound glycoconjugates (glycoproteins, mucins and glycolipids) expressing the globo H or its tetrasaccharide terminus. Recognizes an epitope consisting of the tetrasaccharide Fucα1-2Galβ1-3GalNacβ1-3Gal.
Crossreactivity:	Does not cross-react with mouse. Cross-reactivity with other species has not been tested.
Applications:	Flow Cytometry, ICC, IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:	Flow Cytometry (1-10µg/ml)Immunocytochemistry (1-10µg/ml)Immunohistochemsitry (frozen sections, 1-10µg/ml)Immunohistochemistry (paraffin sections, 1-10µg/ml, no need of antigen retrieval)Western Blot (1-10µg/ml, for glycoproteins)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Application Notes:	Not recommended for immunoprecipitation.
Purity Detail:	Purified from concentrated hybridoma cell culture supernatant by thiophilic adsorption chromatography.
Formulation:	Liquid. In 50mM PBS, pH 8.0, containing 150mM sodium chloride. Contains no sodium azide.
Handling:	Avoid freeze/thaw cycles.
Shipping:	Shipped on Blue Ice
Long Term Storage:	+4°C

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