Product Specification:
| Clone: | 7D11 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Recombinant humanβ-catenin. |
| UniProt ID: | P35222 |
| Species reactivity: | Human, MouseDog |
| Specificity: | Recognizes the N-terminus (exon 2) of human, mouse, rat (weak) and dog β-catenin. |
| Applications: | ELISA, IHC, IP, WB |
| Recommended Dilutions/Conditions: | Western Blot (0.5µg/ml for HRPO/ECL detection)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Positive Control: | Included. (Prod. No. ALX-840-027) |
| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Protocol: | Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. |
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