The Carmovirus-Pelarspovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera of the Tombusviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods.
This test may also detect members of the Tombusvirus and Dianthovirus genera. For a full list of confirmed and predicted reactions, click here.
The Carmovirus-Pelarspovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
User GuideTech Note
This test reacts with a variety of viruses from the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera. The list below represents Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus species that have been experimentally detected with our Carmovirus-Pelarspovirus Group PCR and confirmed by sequencing. If you have confirmed detection of a Alphacarmovirus, Betacarmovirus, Gammacarmovirus, or Pelarspovirus not on this list, please contact us. For viruses predicted to be detected, click here.
| Confirmed Detections | |
|---|---|
| Angelonia flower break virus (AnFBV) | Calibrachoa mottle virus (CbMV) |
| Cardamine chlorotic fleck virus (CCFV) | Carnation mottle virus (CarMV) |
| Cowpea mottle virus (CPMoV) | Elderberry latent virus (ELV) |
| Hibiscus chlorotic ringspot virus (HCRSV) | Melon necrotic spot virus (MNSV) |
| Pelargonium chlorotic ring pattern virus (PCRPV) | Pelargonium flower break virus (PFBV) |
| Pelargonium line pattern virus (PLPV) | Pelargonium ringspot virus (PelRSV) |
| Saguaro cactus virus (SgCV) | Turnip crinkle virus (TCV) |
| Maize chlorotic mottle virus (MCMV)* | Tobacco necrosis virus A (TNV-A)** |
*This is a Machlomovirus.
**This is an Alphanecrovirus.
This test reacts with a variety of viruses from the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera. The list below represents Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus species that have not been experimentally detected, but based on in silico analysis, will likely be detected with our Carmovirus-Pelarspovirus Group PCR. If you have samples of these pathogens below, please contact us.
| Predicted Detections | |
|---|---|
| Clematis chlorotic mottle virus (ClCMV) | Galinsoga mosaic virus (GAMV) |
| Honeysuckle ringspot virus (HRSV) | Japanese iris necrotic ring virus (JINRV) |
| Nootka lupine vein clearing virus (NLVCV) | Pea stem necrosis virus (PSNV) |
| Rosa rugosa leaf distortion virus (RRLDV) | Soybean yellow mottle mosaic virus (SYMMV) |
| Trailing lespedeza virus 1 (TLV1)* | |
*This is an unassigned member of the Procedovirinae subfamily.
Includes:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
- Tech Note
