Test Label: Biotin labeled primer / FAM probeTest Format: AmplifyRP® XRTInternal Control: Yes - Exogenous
AmplifyRP XRT for Foc TR4 is a rapid DNA amplification and detection platform designed for testing banana root, rhizome, stem, pseudostem, and leaf for Fusarium oxysporum f. sp. cubense tropical race 4 (Fusarium odoratissimum), the causal agent of Panama Disease. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify Foc TR4 DNA and an exogenous DNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal DNA amplification and detection system that rapidly amplifies small portions of DNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
Limit of Detection (LOD): 25 pg of genomic DNA; 40 copies of DNA fragments per reaction
Specificity:
- Inclusive: Designed to detect all Fusarium oxysporum f. sp. cubense tropical race 4 strains. Strains detected include: NRRL 36114 (VCG 01213), NRRL 54006 (Foc-TR4 reference strain Focll5) and 5 isolates from VCG 01213/16.
- Exclusive:
- Does not detect: Amplicon DNA fragments from homologous genes of Foc-R1, R2, R3 and STR4. Fungal species Colletotrichum gloeosporioides, Fusarium monoliforme (NRRL 13911), Fusarium pallidoroseum (NRRL 20425), Fusarium solani (NRRL 22587), Fusarium subglutinans (NRRL 25734), Rhizoctonia solani (NRRL 66082, DSM 843, DSM 852, DSM 22843, DSM 22847, DSM 62716, DSM 63002), Fusarium oxysporum f.sp. cubense STR4, Fusarium oxysporum f.sp. cubense R1, Phytophthora citrophthora, Phytophthora heveae, Phytophthora palmivora, Phytophthora kernoviae, Phytophthora cryptogea, Phytophthora ramorum, Pythium aphanidermatum, Pythium irregulare, Pythium ultimum,
- Predicted not to detect via insilico data: Botryosphaeia dothoidea, Colletotrichum gleosporioides (including musae), Alternaria alternata, Cladosporium musae, Mychosphaerella fifienses, Mycorrhizae spp, Xanthomonas spp, or Ralstonia spp.
- No reaction to healthy Banana root, rhizome, stem, pseudostem and leaf.
- Technical Information
- Includes
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.
Includes:
- XRT reaction pellets for Foc TR4 (48)
- Pre-filled 100 µL PD1 Pellet diluent tubes (48)
- AMP1 extraction buffer (55 mL)
- Sample bag, mesh (50)
- User Guide
NOTE: This assay requires a fluorometer to work properly.
