Description
Vitronectin Polyclonal Antibody
Affinity’s Vitronectin Polyclonal Antibody is the base level of our Vitronectin antibody family. The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum. This Vitronectin Polyclonal Antibody is generally intended for use in applications such as immuno-precipitation, immuno-electrophoresis, immuno-depletion and activity neutralization assays.
Product Code: SAVN-IG
Retail Product Size: 10mg vial
Host Animal: Sheep Anti-Human Vitronectin Polyclonal Antibody
Species Cross Reactivity: View Chart
Product Datasheet: Vitronectin Polyclonal Antibody, purified anti-human sheep IgG
Description of Vitronectin (VN)
Vitronectin (VN), previously known as serum-spreading factor or S-protein, is a plasma and serum glycoprotein with a normal concentration ranging from 200 – 400 ug/ml. It exists in both a 75 kDa single-chain form and a 65 + 10 kDa two-chain form. Vitronectin can exist in a least two different conformational forms. The majority of VN found in the circulation is present in the native (“closed”) form. In this form, most of the binding sites for other ligands are cryptic. The second form of VN, the denatured (“open”, multimeric) form, is a result of a conformational change in the native protein induced by denaturants such as urea, adsorption onto surfaces, low pH or reduction and alkylation. This conformational change leads to exposure of the heparin binding site, formation of disulfide-bonded multimers and rupture of the disulfide bond that links the 10 kDa light chain to the 65 kDa heavy chain of the two chain form. The liver is the primary site of VN synthesis, however, Vn is also found in platelets, megakaryocytes, monocytes and macrophages. VN plays an important role in a number of physiological and pathophysiological processes. It promotes the adhesion and spreading of a wide variety of cell types and is a subcomponent of the soluble SC5b-9 complex of complement where it protects bystander cells from cytolysis. VN also plays an important role in fibrinolysis by stabilizing PAI-1 in its active conformation which otherwise rapidly converts to a latent form.1-3
References and Reviews
- Tomasini, B.R., and Mosher, D.F. Vitronectin. Prog. Hemost. Thromb., 10:269-305, 1991.
- Hess, S., Stockmann, A., Voler, W., and Preissner, K.T. Multimeric vitronectin: structure and function. In: Biology of Vitronectins and their Receptors, Elsevier Science Publishers, Amsterdam, p. 21-29, 1993.
- Preissner, K.T., and Jenne, D. Vitronectin: a new molecular connection in haemostasis. Thrombo. Haemost., 66(2):189-194, 1991
