Features: - Ready-to-Use - Mouse and Rabbit IgG primary antibody detection- Peroxidase enzyme detection system - Advanced Elite® Avidin/Biotin technology - Highest sensitivity, low background - Approximately 5 times more sensitive - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 250-500 sections Kit Contents: - 50 ml Normal Horse Serum - 50 ml Biotinylated Horse Anti-Mouse and Rabbit IgG - 50 ml Stabilized Elite® ABC Reagent(Elite® Avidin DH and Biotinylated Peroxidase)Applications: - Immunohistochemistry- Immunocytochemistry - In situ hybridization- Blotting Applications- Elispot- ELISADetails:The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology:The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target.The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol.Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1018).Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting