Features:- Complete staining kits- Rabbit IgG primary antibody detection - Peroxidase enzyme detection system- ImmPRESS® Amplified polymer technology (HRP)- Convenient prediluted, ready-to-use reagents- Highest sensitivity, low background- Sufficient reagents to stain approximately 75-150 (15 ml kit) or 250-500 (50 ml kit) sectionsKit Contents:- BLOXALL™ Endogenous Enzyme Blocking Solution (ready-to-use)- 2.5% Normal Horse Serum (ready-to-use)- Amplifier Antibody (goat anti-rabbit Ig, ready-to-use)- ImmPRESS® Excel Polymer Reagent (horse anti-goat Ig, HRP, ready-to-use)- ImmPACT™ DAB EqV Reagent 1 (Chromogen)- ImmPACT™ DAB EqV Reagent 2 (Buffer)Applications:- Immunohistochemistry- Immunocytochemistry- In situ hybridization- ELISADetails:The ImmPRESS® Excel Amplified HRP (peroxidase) Polymer Staining Kit is an enzymatic, amplification system that produces crisp, highly sensitive, specific staining with low background. The ImmPRESS® Excel Amplified Staining System capitalizes on all the advantages of the ImmPRESS® polymerized reporter enzyme staining system technology but offers additional sensitivity and convenience.This system employs a proprietary ready-to-use (RTU) Amplifier Antibody, followed by a ready-to-use (RTU) ImmPRESS® Excel HRP Polymer Reagent. These reagents are affinity purified and extensively cross-adsorbed to ensure high sensitivity and low background. In addition, the ImmPRESS® Excel Reagent is conjugated to horseradish peroxidase micropolymers, a novel approach that avoids the use of large dextrans or other macromolecules as a backbone. These unique micropolymers allow a higher density of enzymes per antibody to bind the target with minimal interference. The result is an increase in target accessibility, signal intensity, and significantly less background staining.The included ImmPACT™ DAB EqV Substrate produces a crisp, dark brown reaction product with the excellent sensitivity and low background characteristic of the ImmPRESS® /ImmPACT™ combination.
DocumentsDatasheet
Enzyme Substrate Combinations Chart
Guide to Multiple Antigen Labeling
SDS
Troubleshooting Guide
VECTASTAIN ABC System Overview
RestrictionsFor research use only
Available for shipment within the USA only
Related ProductsImmPRESS®-AP Anti-Rabbit IgG (Alkaline Phosphatase) Polymer Detection Kit - LS-J1053
ImmPRESS® HRP Anti-Rabbit IgG (Peroxidase) Polymer Detection Kit, made in Horse - LS-J1060
ImmPRESS® HRP Anti-Rabbit IgG (Peroxidase) Polymer Detection Kit, made in Goat - LS-J1066
ImmPRESS® HRP Universal Antibody (Anti-Mouse IgG/Anti-Rabbit IgG, Peroxidase) Polymer Detection Kit, made in Horse - LS-J1068
ImmPRESS® Duet Double Staining HRP/AP Polymer Kit (anti-rabbit IgG-brown, anti-mouse IgG-red) - LS-J1071
LifeSpan成立于1995年,已经成为分子病理学领域的领导品牌,专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体,定制的免疫组化研究服务,超过500种蛋白研究用人免疫组化材料,及组化和病理学服务;提供2,000,000样本,包括冰冻及石蜡切片的正常和疾病组织,进行对外研究服务。对外服务项目范围广泛,由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。
产品评述样例
为了研究表达增加的HO-1在脑干死亡中的影响,采用了Lifespan Biosciences的兔抗HIF-1beta多克隆抗体进行了免疫中和干预免疫印迹实验。
为了研究PROK1通过PROKR1和calcineurin/NFAT信号通路对IL11表达的调节,采用了Lifespan Biosciences的抗PROKR1抗体进行免疫组织化学实验。
为了揭示蝰毒素如何促进感染,采用Lifespan Biosciences的抗HADHB抗体进行免疫印迹试验。
为了建立能够特异的检测抗HCV化合物的报告系统,使用了LifeSpan BioSciences公司的NS3羊多抗来进行免疫印记实验。
为了研究静态成纤维细胞的作用使用了Lifespan Biosciences公司的抗IDH1抗体来进行蛋白印迹实验。
为了阐明源于E3泛素连接酶(TRIM32)突变的肌肉萎缩症包含神经性的和肌原性的特征,采用了Lifespan Biosciences公司的兔抗TRIM32抗体(1:1000),进行了蛋白质印迹实验。
使用LifeSpan生物公司的抗FPRL1抗体(1:100)进行流式细胞计数来研究促炎症反应多肽LL-37在卵巢肿瘤进展中的作用。
Lifespan Biosciences公司P2X7抗体可用于免疫组化实验。来研究P2X7受体在角膜上皮损伤修复中的作用。
产品样例
IHC-plus™ GPR21 Antibody (C-Terminus) LS-A108
IHC-plus™ CCR10 / GPR2 Antibody (N-Terminus) LS-A1153
IHC-plus™ CHRM3 / M3 Antibody (Cytoplasmic Domain) LS-A1421
IHC-plus™ GPR32 Antibody (Cytoplasmic Domain) LS-A1679
IHC-plus™ GPR61 Antibody (C-Terminus) LS-A1688
IHC-plus™ VIPR1 Antibody (N-Terminus) LS-A3589
IHC-plus™ GPR182 / ADMR Antibody (N-Terminus) LS-A390
IHC-plus™ RAIG2 / GPRC5B Antibody (C-Terminus) LS-A4088
IHC-plus™ TACR2 / NK2R Antibody (C-Terminus) LS-A4320
IHC-plus™ GPR52 Antibody (Cytoplasmic Domain) LS-A444