品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Advanced immunochemical / Advanced immunochemical/Monoclonal mouse anti-D-dimer/0.2 mg/2-DD1
商品描述
General Category:
- Monoclonal antibodies » Blood Coagulation and AnemiaMonoclonal antibodies » Cardiac Markers » D-dimer
Disease Category:
- Cardiac Markers » Monoclonal antibodies » D-dimerBlood Coagulation and Anemia » Monoclonal Antibodies
MAbs:
DD1, DD2, DD3, DD4, DD5, DD6, DD22, DD41, DD44, DD46, DD93New MAbs: DD189. DD255Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Specificity:
All MAbs recognize D-dimer and high molecular weight fibrin degradation products.MAb DD93 recognizes a cross-linked region of D-dimer.MAbs DD1, DD2, DD3, DD22, DD41, DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen.MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.
MAb Isotypes:
IgG1 for MAbs DD93, DD189, DD255IgG2a for MAbs DD1, DD6, DD22, DD41, DD46IgG2b for MAbs DD2, DD3, DD4, DD5, DD44
Applications:
Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products.All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 and DD189 interact with beta-chain of D- dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gammachain of D-dimer in Western blotting under reducing conditions.Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in humanplasma (capture-detection):One-step sandwich immunoassays:DD189 – DD255 (Equal specificity to D-dimer and high MW fibrin degradation products). DD2 –DD41 (Slightly more specific to high MW fibrin degradation products at high concentrations).Two-step sandwich immunoassays*:DD2 – DD4 (Approximately equal specificity to D-dimer and high MW fibrin degradation products).*To be analysed in a two-step sandwich immunoassay, plasma must be diluted 1:1 or more with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.)
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage Conditions:
+ 4 °C
Material Safety Note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Additional product
information:
D-dimer and High Molecular Weight Fibrin Degradation Products Assay Notes
References:
Search D-dimer antibody references fromPubMed.
