CII-11, CII-29, CII-55, CC24, CC27, CC34Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.

IgG1 for MAbs CII-11, CII-29, CII-55, CC24, CC27, CC34

All MAbs recognize rat C-peptide in ELISA and Sandwich type immunoassay. C-peptide in ELISA and Sandwich type immunoassay.MAbs can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples. See News Letter for more information.Best pairs for sandwich immunoassays. For sandwich immunoassay the general recommendation is to use combinations of MAbs named as “CC” (MAbs specific to C-terminal part of rat C-peptide) and MAbs named as ”CI” or “CII” (MAbs specific to N-terminal part of rat C-peptide).The most sensitive pairs recommended for sandwich-type rat C-peptide immunoassays are (capture-detection):rat C-peptides I and II immunodetectionCII-29 – CC24CII-55 – CC24CC34 – CII-11CC27 – CII-29

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

TThis product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Rat C-peptide and proinsulin Assay Notes

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