Bovine brain tissue.

S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kDa. In human brain tissue S-100 protein is mainly presented by two isoforms – ββhomodimer (S-100b) and αβ heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, in diagnosis and prognosis of clinical outcome in acute stroke. S-100 protein is suitable for use as a standard in immunoassay, as an immunogen for antiserum production and tracer for iodination.

Purity > 95 %. After native gel electrohoresis by Ornstein-Davis S-100 protein is presented by two bands corresponding to αβ- and ββ-forms.S-100 protein concentration was determined by Lowry method using BSA as a standard.

Lyophilized from 5 mM Tris-HCl, pH 7.5 with 5 mM 2-mercaptoethanol and 5 mM EDTA.Product is recommended to reconstitute with 5 mM 2-mercaptoethanol.

-20 °C (between -15 and -30 °C allowed)

Avoid repeated freezing and thawing.It is recommended to aliquot the product after reconstitution. This product is available only in research amounts.

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.