ACROBiosystems/Human APRIL / TNFSF13 Protein, Fc Tag/1mg (250ug × 4)/APL

价格
¥46200.00
货号:APL
浏览量:127
品牌:ACROBiosystems
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商品描述
  • Synonym
    APRIL,TNFSF13,TALL-2,TRDL-1,CD256,TALL2,ZTNF2
  • Source
    Human APRIL, Fc Tag (APL-H5267) is expressed from human 293 cells (HEK293). It contains AA Ala 105 - Leu 250 (Accession # O75888-1).
    Predicted N-terminus: Pro
    Request for sequence
  • Molecular Characterization
    Online(Ala 105 - Leu 250) O75888-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 42.8 kDa. The protein migrates as 48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human APRIL, Fc Tag (Cat. No. APL-H5267) SDS-PAGE gel

Human APRIL, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human APRIL, Fc TagHuman APRIL, Fc Tag (Cat. No. APL-H5267) ELISA bioactivity

Immobilized Human BCMA, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BCA-H5253) at 5 μg/mL (100 μL/well) can bind Human APRIL, Fc Tag (Cat. No. APL-H5267) with a linear range of 0.6-10 ng/mL (QC tested).

Protocol
Human APRIL, Fc TagHuman APRIL, Fc Tag (Cat. No. APL-H5267) ELISA bioactivity

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Protocol
  • Background
    APRIL(a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

ACROBiosystems治疗性抗体的功效不仅取决于Fab片段及其与靶抗原的结合活性,还取决于Fc片段及其与关键Fc受体的相互作用。Fc片段对FcRn的结合亲和力(FCGRT&B2M)将预测抗体的半衰期,而Fc片段与FcγRIIIa(CD16a)之间的结合亲和力将影响抗体引发ADCC的能力(抗体依赖性细胞介导的细胞毒性)。因此,必须在抗体工程化过程中针对一组受体测试候选人。ACROBiosystems提供了完整的重组Fc受体蛋白集合,包括它们的常见变体,以帮助您加快mAb的开发。