- SynonymGPNMB,HGFIN,NMB,Osteoactivin
- SourceHuman Osteoactivin, His Tag (GPB-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Pro 486 (Accession # AAH32783).Predicted N-terminus: Ala 22Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 53.4 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Osteoactivin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- Citations
Osteoactivin regulates head and neck squamous cell carcinoma invasion by modulating matrix metalloproteases
Authors: Arosarena OA, et al.
Journal: J Cell Physiol 2017
Application: Cell culture
Request for Full-text
- BackgroundTransmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
- References
- (1)Weterman MA., et al., 1995, International Journal of Cancer 60 (1): 73–81.
- (2)Abdelmagid, SM., et al., 2008, Exp Cell Res 314 (13): 2334–51.
- (3)Abdelmagid SM., et al., 2007, J Cell Physiol. 210 (1): 26–37.
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