- SynonymNLGN3,Neuroligin-3,Gliotactin homolog,KIAA1480,NL3
- SourceHuman Neuroligin 3, His Tag (NL3-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Ser 689 (Accession # Q9NZ94-2(S92Y,G178C,P642S)).Predicted N-terminus: Gln 38Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 74.4 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Neuroligin 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundNeuroligin 3 (NLGN3),a single-pass type I membrane protein ,is also known as gliotactin homolog and KIAA1480, which belongs to the type-B carboxylesterase/lipase family. As the cell surface protein,NLGN3 is involved in cell-cell-interactions via its interactions with neurexin family members. NLGN3 also plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. NLGN3 probably can promote the initial formation of synapses, but is not essential for this. Furthermore,NLGN3 may also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.
- References
- (1)Graf E.R., et al., 2004, Cell 119:1013-1026.
- (2)Suckow A.T., et al., 2008, Endocrinology 149:6006-6017.
- (3)Jamain S., et al., 2003, Nat. Genet. 34:27-29.
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