- SynonymMYOC,Myocilin,GLC1A,GPOA,JOAG,JOAG1,TIGR
- SourceHuman Myocilin, His Tag (MYC-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 33 - Met 504 (Accession # NP_000252).Predicted N-terminus: Arg 33Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 54.3 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE) due to most of the MYOC was cleaved at Glu 214, generating a C-terminal fragment.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human Myocilin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundMyocilin, trabecular meshwork inducible glucocorticoid response is also known as MYOC, GLC1A, GPOA, JOAG, JOAG1, TIGR. Myocilin / MYOC contains one olfactomedin-like domain. Myocilin is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.
- References
- (1)Young H. Kwon., et al., 2009, N Engl J Med, 360:1113-24.
- (2)Torrado, Mario., et al., 2002, Hum. Mol. Genet. (England) 11 (11): 1291–301.
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