- SynonymCKB,B-CK,CKBB
- SourceHuman CKB, His Tag (CKB-H5143) is expressed from E.coli cells. It contains AA Pro 2 - Lys 381 (Accession # AAH19259).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 43.5 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CKB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundCreatine kinase B-type (CKB) is also known as Creatine kinase B chain, B-CK and CKBB. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Furthermore, ectopic expressionof the CKB in red cells and platelets is a rare, benign anomaly detected during a newborn screening program for Duchenne muscular dystrophy.
- References
- (1)Daouk G.H., et al., 1988, J. Biol. Chem. 263:2442-2446.
- (2)Olsen J.V., et al., 2010, Sci. Signal. 3:RA3-RA3.
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