- SynonymTNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270
- SourceHuman HVEM, Fc Tag (HVM-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # NP_003811.2).Predicted N-terminus: Leu 39Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehaloseis added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 2 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag (Cat. No. HVM-H5258) with a linear range of 0.3-20 ng/mL (QC tested).
Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag (Cat. No. BTA-H82F8) with a linear range of 0.156-5 μg/mL (Routinely tested).
Loaded Human HVEM, Fc Tag (Cat. No. HVM-H5258) on Protein A Biosensor, can bind Human CD160, His Tag (Cat. No. BY5-H5229) with an affinity constant of 0.44 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- BackgroundHerpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily.HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM.Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.
- References
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