- SynonymCA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16
- SourceBiotinylated Human CA125, Fc,Avitag (CA5-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1).Predicted N-terminus: Gly 12660Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in TriswithGlycine,ArginineandNaCl,pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human CA125, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag (Cat. No. CA5-H82F4) with a linear range of 2-78 ng/mL (QC tested).
- BackgroundThe CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.
- References
(1)O'Brien TJ., etal. 2002, Tumour Biol., 23(3), 154-69.
(2)O'Brien TJ., etal. 1998, Int J Biol Markers., 13(4), 188-95.
(3)Yin BW., etal. 2001, J Biol Chem., 276(29), 27371-5.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
