Details

Source

Thermus flavus

Applications

• Efficiently synthesizes DNA at elevated temperatures (1)
• Can be used for amplification of nucleic acids in PCR and LCR reaction
• Used for high temperature DNA sequencing
• Broader Mg2+ ions concentration tolerance than Taq DNA polymerase

Reagents Supplied

10X Tfl DNA Polymerase Reaction Buffer

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes

Concentration

3.0 units/µl

Storage Buffer

50 mM Tris HCl (pH 7.5)0.1 mM EDTA5 mM dithiothreitol50% (v/v) glycerolStabilizers

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)50 mM potassium chloride10 mM MgCl₂1 mM dithiothreitol1 mg/ml bovine serum albumin300 µg/ml activated calf thymus DNA0.2 mM of each dCTP, dGTP, dTTP and [α-³²P]dATPIncubation is for 10 minutes at 70°Cin a reaction volume of 50 µl

Storage Conditions

Store at -20°CProduct shipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF

References

1. Kaledin, A. S., Sliusarenko, A.G. and Gorodetskii, S. I. (1980) Biokhimiya 45, 644-651

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