Details

Source

Bacillus stearothermophilus H3

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

50°C

Heat Inactivation

80°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂ 100 mM NaCl1 mM DTT100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 100%*Buffer 2: 100%*Buffer 3: 100%Buffer 4: 75%Buffer 5: 50%*star activity

Assay Conditions

See Certificate of Analysis Below

Storage Buffer

10 mM Tris-HCl (pH 7.5) 50 mM KCl0.1 mM EDTA1 mM dithiothreitol0.02% Triton X-100™500 µg/ml bovine serum albumin 50% (v/v) glycerol

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificates of AnalysisLot 2203011 PDF

MSDSMSDS PDF

>>> 更多资讯详情请访问蚂蚁淘商城