Details

Source

Micrococcus luteus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1:25%Buffer 2: 75%Buffer 3: 100%Buffer 4: 50%Buffer 5: 50%*Star activity

Storage Buffer

50 mM Tris‑HCl (pH 7.4 at 4°C) 50 mM NaCl0.1 mM EDTA1 mM dithiothreitol500 µg/ml bovine serum albumin0.1 % Triton X-100™50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100µg/ml bovine serum albumin1 μg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Anaysis PDF-Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases

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