Details

Source

Bacillus globigii RUB 561

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂100 mM NaCl1 mM dithiothreitol100 μg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%Buffer 2: 100%Buffer 3: 100%Buffer 4: 50%Buffer 5: 50%*star activity

Storage Buffer

20 mM Tris‑HCl (pH 7.5 at 4°C)200 mM NaCl10 mM MgCl₂1 mM dithiothreitol0.1 mM EDTA500 µg/ml bovine serum albumin0.1% (v/v) Triton X-100™50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificates of AnalysisLot 2406007 PDF

MSDSMSDS PDF

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