Details
Rsa I Source
Rhodopseudomonas sphaeroides
Reagents Supplied
CMX Buffer 1
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 1:10 mM Tris-HCl (pH 7.0 at 37°C)10 mM MgCl21 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1:100%Buffer 2:75%Buffer 3: 25%Buffer 4: 75%Buffer 5: 50%
Storage Buffer
10 mM Tris-HCl (pH 7.4 at 4°C)50 mM KCl0.1 mM EDTA1 mg/ml bovine serum albumin1 mM dithiothreitol0.1 M PMSF50% (v/v) glycerol
Assay Conditions
10 mM Tris‑HCl (pH 7.0 at 37°C) 10 mM MgCl21 mM dithiothreitol100µg/ml bovine serum albumin1 µg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
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