Details
Source
Haemophilus influenzae Rd
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
65°C for 10 minutes
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: <25%Buffer 2: 100%Buffer 3: <25%Buffer 4:50%Buffer 5: <25%*Star activity
Storage Buffer
10 mM Tris-HCl (pH 7.4 at 4°C)200 mM NaCl0.5 mM EDTA1 mM DTT0.5 mg/ml bovine serum albumin5 µg/ml Poly-L-lysine0.1% Triton X-100™50% (w/v) glycerol
Assay Conditions
50 mM Tris‑HCl (pH 7.5 at 37°C) 10 mM MgCl250 mM NaCl1 mM dithiothreitol1 µg/ml bovine serum albumin1 µg lambda DNAReaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
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