Details
Sma I Source
Serratia marcescens
Reagents Supplied
CMX Buffer 4
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of Adenovirus 2 DNA in 1 hour at 30°C in a total reaction volume of 50 μl
Star Activity
Yes
Incubation Temperature
30°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 4:20 mM Tris-Acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate100 µg/ml bovine serum albumin1 mM dithiothreitol
Activity in Buffers
Buffer 1: 0%Buffer 2:0%Buffer 3: 0%Buffer 4: 100%Buffer 5: 75%*Star activity
Storage Buffer
20 mM Tris-HCl (pH 7.5 at 4°C)1 mM EDTA7 mM β-mercaptoethanol500 μg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
20 mM Tris‑acetate (pH 7.5 at 37°C)50 mM potassium acetate10 mM magnesium acetate100 µg/ml bovine serum albumin1 μg Adenovirus 2 DNA1 mM dithiothreitol
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
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