Details
Source
Bacillus stearothermophilus HKCNote: Purified from E coli strain that carries the cloned bsiHKCRI gene.
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl
Star Activity
Yes
Incubation Temperature
55°C
Heat Inactivation
No
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C)100 mM NaCl10 mM MgCl21 mM DTT100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: <25%Buffer 2: 75%Buffer 3: 100%Buffer 4: 75%Buffer 5: 50%*star activity
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 4°C)50 mM KCl1 mM dithiothreitol0.1 mM EDTA500µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
See Certificate of Analysis Below
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of AnalysisLot 2206007 PDF
MSDSMSDS PDF
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