Details
PinA I Source
Pseudomonas inaequalis
Reagents Supplied
CMX Buffer 5
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in total reaction volume of 50 µl
Star Activity
No
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 5:20 mM Tris-HCl (pH 7.0 at 37°C)5 mM MgCl250 mM KCl1 mM dithiothreitol0.1 mg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 50%Buffer 2:75%Buffer 3: 50%Buffer 4: 50%Buffer 5: 100%
Storage Buffer
20 mM Tris-HCl (pH 7.5 at 4°C)0.1 mM EDTA100 mM NaCl10 mM β-mercaptoethanol0.05% (v/v) Triton X-100™50% (v/v) glycerol
Assay Conditions
20 mM Tris‑HCl (pH 7.0 at 37°C) 5 mM MgCl250 mM KCl 1 mM dithiothreitol100µg/ml bovine serum albumin1 µg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction Endonucleases
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