Details

Nde I Source

Neisseria denitrificans

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1:50%Buffer 2:100%Buffer 3: 75%Buffer 4: 100%Buffer 5: 75%*Star activity

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)0.1 mM EDTA1 mM DTT50 mM KCl500 μg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

20 mM Tris‑acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol100µg/ml bovine serum albumin1 µg lambda DNA

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases

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