Details
Taq II Source
Purified from E.coli strain that carries the cloned taqRII gene from Thermus aquaticus
Unit Definition
One unit is the amount of enzyme required to digest 1 µg of pBR322 DNA to obtain stable digestion pattern in 1 hour in a total reaction volume of 20 µl
Star Activity
N/A
Reaction Temperature
65°C
Heat Inactivation
No
Assay Unit Substrate
pBR322
Reaction Buffer
50 mM Tris-HCl (pH 8.5 at 25°C)10 mM MgCl21 mM dithiothreitol
Notes:
Storage Buffer
20 mM Tris-HCl (pH 7.5 at 25°C)0.1 mM EDTA200 mM NaCl1 mM dithiothreitol200 µg/ml bovine serum albumin0.02% Triton X-100™0.02% Tween 20™50% (v/v) glycerol
Assay Conditions
50 mM Tris-HCl (pH 8.5 at 25°C)10 mM MgCl21 mM dithiothreitol1µg pBR322
Taq II Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF - Current LotMSDS PDF - Current Lot
Categories
Restriction Endonuleases
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