Details
Source
Xanthomonas badrii
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Reaction Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl21 mM dithiothreitol50 mM NaCl100 µg/ml bovine serum albuminNote: blocked by overlapping Dam methylation
Activity in Buffers
Buffer 1: 25%Buffer 2:100%Buffer 3: 100%Buffer 4: 75%Buffer 5:50%*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.4 at 4°C)0.1mM EDTA1 mM dithiothreitol500 µg/ml BSA50% (v/v) glycerol
Assay Conditions
10 mM Tris‑HCl (pH 7.5 at 37°C) 10 mM MgCl250 mM NaCl 1 µg of Adenovirus 2 DNA1 mM dithiothreitol100µg/ ml bovine serum albuminIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of Analysis PDF - Current LotMSDS PDF- Current Lot
Categories
Restriction Endonucleases
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