Details

Source

Purified from an E coli strain that carries the DpnI gene from Dipplococcus pneumoniae

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1μg of pBR322 dam methylated DNA in 1 hour in a total reaction volume of 50μl

Star Activity

No

Inactivation Temperature

80°C for 10 minutes

Incubation Temperature

37°C

Assay Unit Substrate

pBR322 dam methylated DNA

Reaction Buffer

CMX Buffer 4:20 mM Tris-acetate (pH 7.5 at 37°C)10 mM magnesium acetate50 mM potassium acetate1 mM dithiothreitol

Activity in Buffers

Buffer 1: 75%Buffer 2: 100%Buffer 3: 100%Buffer 4:100%Buffer 5: 0%

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)400 mM NaCl0.1 mM EDTA1 mM dithiothreitol0.1% Trition X-100™200 μg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Notes

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of AnalysisLot 2407019 PDF

MSDSMSDS PDF

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