Details
SspISource
Sphaerotilusspecies
ReagentsSupplied
CMXBuffer2
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µgoflamBDaDNAin1hourat37°Cinatotalreactionvolumeof50μl
StarActivity
Yes
ReactionTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer2:
10mMTris-HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
ActivityinBuffers
Buffer1: 50%
Buffer2: 100%
Buffer3:50%
Buffer4:50%
Buffer5:50%
*Staractivity
StorageBuffer
10mMTris-HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
1mMdithiothreitol
500µg/mlbovineserumalbumin
0.2%TritonX-100™
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.5at37°C)
10mMMgCl2
50mMNaCl
1mMdithiothreitol
100µg/mlbovineserumalbumin
0.025%TritonX-100™
1µglambdaDNA
Reactionvolume50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF-CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases