Details
RsaISource
Rhodopseudomonassphaeroides
ReagentsSupplied
CMXBuffer1
UnitDefinition
Oneunitistheamountofenzymerequiredtocompletelydigest1µglamBDaDNAin1hourinatotalreactionvolumeof50µl
StarActivity
No
IncubationTemperature
37°C
HeatInactivation
10minutesat65°C
AssayUnitSubstrate
LambdaDNA
ReactionBuffer
CMXBuffer1:
10mMTris-HCl(pH7.0at37°C)
10mMMgCl2
1mMdithiothreitol
100µg/mlbovineserumalbumin
ActivityinBuffers
Buffer1: 100%
Buffer2: 75%
Buffer3:25%
Buffer4:75%
Buffer5:50%
StorageBuffer
10mMTris-HCl(pH7.4at4°C)
50mMKCl
0.1mMEDTA
1mg/mlbovineserumalbumin
1mMdithiothreitol
0.1MPMSF
50%(v/v)glycerol
AssayConditions
10mMTris‑HCl(pH7.0at37°C)
10mMMgCl2
1mMdithiothreitol
100 µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl
StorageConditions
Storeat-20°C
Shippedondryice
Downloads
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
Categories
RestrictionEndonucleases