| IAP p60 from Listeria Monocytogenes; Invasion-associated Protein p60 from Listeria Monocytogenes |
| Monoclonal Antibody |
| p6007 |
| Mouse IgG1κ |
| Recombinant Listeria monocytogenes p60. |
| ELISA: (direct or indirect: 1:5000-1:10000)Western blot: (1:2000-1:10000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).Optimal conditions should be determined individually for each application. |
| Listeria monocytogenes |
| Recognizes L. monocytogenes p60 protein. Does not cross-react with other Listeria species. |
| Protein G-affinity purified. |
| 1mg/ml |
| Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| BLUE ICE |
| +4°C |
| -20°C |
| After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles. |
| Stable for at least 1 year after receipt when stored at -20°C. |
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Listeria monocytogenes is a intracellular bacterium that is the causative agent of Listeriosis. It is one of the most virulent foodborne pathogens. Listeria species are known to secrete a 60-kDa protein collectively termed p60, which is encoded by the iap (invasion-associated-protein) gene and secreted in large quantites into the growth media. p60 is a highly immunogenic murein hydrolase that is essential for cell division.
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