주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
X-α-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4- based two-hybrid interactions directly on agar (Figure 1). This quick and easy assay eliminates the need for time-consuming β-galactosidase liquid and filter-lift assays in two-hybrid analysis. The X-α-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in two-hybrid analysis. MEL1 encodes the secreted enzyme α-galactosidase, which hydrolyzes colorless X-α-Gal into a blue end-product. Yeast colonies that express α-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-α-Gal (Table 1).
Figure 1. X-α-Gal detects secreted α-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.
□ Storage Conditions
-20°C
□ References
1. Aho, S., et al. (1997) Anal. Biochem. 253:270-272.2. Liljestrom, P. L. (1985) Nucleic Acids Res. 13:7257-7268.
