c-Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The Myc protein activates transcription as part of a heteromeric complex with Max. Myc protein represses the expression of Ferritin-H, which sequesters intracellular iron, and stimulates the expression of IRP2 (Iron Regulatory Protein-2), which increases the intracellular iron pool. Myc induces transcription of the E2F1, E2F2 and E2F3 genes. Myc-induced S phase and apoptosis requires distinct E2F activities. The induction of specific E2F activities is an essential component in the Myc pathways that control cell proliferation and cell fate decisions.

Western blot analysis: 2-4 µg/ml

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