Abeomics蚂蚁淘在线

商品

品牌

文章

热门搜索：

快报

蚂蚁淘特色产品FLOWTECH造影剂蚂蚁淘授权代理品牌-德国Emfret 蚂蚁淘旗下188精品现货平台正式上线啦！！！蚂蚁淘四月促销蚂蚁淘获得vitanavitech代理商授权蚂蚁淘获得QA-bio代理商授权蚂蚁淘获得Viagenbiotech代理商授权 polysciences公司24765-1到现货了(内附代理商整理说明书）蚂蚁淘四月团建“环苏州古城一周” Clodronate Liposomes用量更正！

品牌首页品牌新闻品牌简介产品中心品牌咨询联系我们

品牌分类

品牌咨询

联系方式

公司地址

苏州工业园区生物纳米园A4#216

联系电话

4000-520-616 / 18915418616

传真号码

0512-67156496

电子邮箱

info@ebiomall.com

公司网址

https://www.ebiomall.com

蚂蚁淘在线 / 品牌中心 / Abeomics / Abeomics/AP-1 Leeporter™ Luciferase Reporter-HEK293 Cell Line/For Profit/14-102ACL

Abeomics/AP-1 Leeporter™ Luciferase Reporter-HEK293 Cell Line/For Profit/14-102ACL

价格

面议

货号：14-102ACL

浏览量：127

品牌：Abeomics

服务

全国联保

正品保证

正规发票

签订合同

加入收藏

立即购买

商品描述

Description
Product Info
Application Note
Review (0)


Amount :	Non Profit

The AP-1 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activator protein 1 (AP-1). The AP-1 transcription factors are homo- or hetero-dimers that consist of proteins belonging to a group of structurally and functionally related members of the Jun family (c-Jun, JunB and JunD), the Fos (c-Fos, FosB, Fra-1 and Fra-2) and the subfamilies of ATF (ATFa, ATF-2 and ATF-3) and JDP (JDP-1 and JDP-2). The AP-1 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.

Content :	Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :	Immediately upon receipt, store in liquid nitrogen.

Application:

Monitor the AP-1 signaling pathway activity.
Screen for activators or inhibitors of the AP-1 signaling pathway.

Culture conditions:

Cells should be grown at 37^oC with 5%CO₂using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3µg/ml of Puromycin(Note: Puromycin can be omitted during the reporter cell assays).

It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37^oCwater-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in37^oC-CO₂incubator.

Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.

To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.

Functional validation:

A. Response of AP-1 Leeporter™ –HEK293 cells to phorbol 12-myristate 13-acetate (PMA)

1. Harvest AP-1 Leeporter™ –HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100µlof growth medium at 5 x 10⁴ cells/well.

2. Incubate cells at37^oCin aCO₂incubator for overnight.

3. The next day, stimulate cells with different concentrations of PMA.

4. Incubate at37^oCin aCO₂incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

There are currently no product reviews

Write a review on this product!

Customers who purchased this product also purchased
IL-8 Leeporter™ Luciferase R...
NF-kB Leeporter™ Luciferase ...
Human Peripheral Blood CD19/CD...
Human Peripheral Blood CD19 B ...
Human Peripheral Blood CD14 Mo...
Human Peripheral Blood CD8 Cyt...
Human Peripheral Blood CD8/CD4...
Human Peripheral Blood CD4 Hel...
Human Peripheral Blood CD4/CD4...
Human Peripheral Blood CD4/CD4...
Human Peripheral Blood CD4/CD2...
Human Peripheral Blood CD3 Pan...
Quick-blots (Rat Tissues)
Quick-blot (Mouse Tissues)

Most viewed Products

Recombinant Human Thioredoxin-Like ...

Monoclonal antibody to Human PD-L1 ...

Monoclonal Antibody to Caspase-3 (P...

Polyclonal Antibody to Beta actin

Monoclonal Antibody to GAPDH (Clone...

Monoclonal Antibody to Human IL-1be...

Monoclonal antibody to B7-H4 (Clone...

Polyclonal Antibody to Beta Tubulin

Peroxidase conjugated Goat anti Mou...

Monoclonal Antibody to EpCAM (CD326...

Abeomics的人PD-L1重组Fc融合蛋白（正在供货）CHO-K1 Celline。 PD-L1（CD274 / B7-H1）是一种重要的膜结合共刺激分子，属于B7超家族，可通过其受体抑制免疫反应，PD-1和PD-L1在炎症性疾病的发病机理中起关键作用死亡1）。它在单核吞噬细胞系统（MPS）中广泛表达，可共同刺激T细胞并调节炎症反应。PD-L1通过对T细胞功能产生负面共刺激作用来发挥炎症调节功能，以抑制细胞因子分泌，促进活化T细胞凋亡并诱导T细胞无反应。在细菌感染，炎症和许多自身免疫性疾病中，已经报道了CD274的异常表达和失调。

资质认证

获得国家资质，权威认证！
全国联保

全国联保，官方无忧售后
正规发票

正规发票，放心购买
签订合同

签订合同，保障您的权益

/**/