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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
eNOS
nitric oxide synthase 3 (endothelial cell)
Download Product PDFProduct Preparation
A synthetic peptide corresponding to the internal sequence (residing in 550-650aa) of human eNOS protein.This sequence is identical among human, rat, mouse, bovine origins.
Background
NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO.Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be enhanced through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. There are three types of NOS: two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II).iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. The phosphorylation of eNOS by Protein kinase C was associated with an increase in NO production.
PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification | SPECIFICITY This antibody onlyrecognizes ~130 kDa of human eNOS. It does not detect human inducible NOS or rat brain NOS.It also reacts with mouse and rat eNOS protein. The other species are not tested. |
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer. | STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity.Avoid repeated freezing-thawing cycles. |
Gene ID
4324
Applications/Suggested Working Dilutions
Western Blot 0.1-1 µg/ml | ELISA 0.01-0.1 µg/ml | Immunoprecipitation 2-5µg/ml | IHC 2-10µg/ml | Flow cytometry 5-10µg/ml |
