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商品描述
CD136 (RON)
Macrophage stimulating 1 receptor; CD136; RON
Download Product PDFProduct Preparation
A synthetic peptide corresponding to the human CD136 at internal domain. This sequence is identical to mouse or rat origin.
Background
CD136, a transmembrane receptor tyrosine kinase, also known as Macrophage stimulating 1 receptor (MST1R) and RON (Recepteur d’origine nantais), is a member of the MET proto-oncogene family (63% homology), and is the receptor for macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. Binding of MSP to CD136 stimulates tyrosine phosphorylation on Tyr 1238 and Tyr 1239. This phosphorylation leads to a up-regulation of CD136 catalytic activity and subsequent activation of downstream signaling molecules. In addition, CD136 contains putative autophosphorylation sites on Tyr 1353 and Tyr 1360. RON mediates multiple signaling cascades, such as MAPK, PI3K/AKT, FAK pathways. CD136 activation can also significantly increase C-Src activity. CD136 is a disulfide-linked 180kD heterodimer composed of a 35kD alpha chain and a 150kD beta chain, and is mainly expressed in cells of epithelial origin. CD136 is thought to play a role in early embryonic development and in the inflammatory response as well as implicated in the progression and metastasis of tumors including those of the breast and colon.
PURIFICATION The Rabbit IgG is purified by Epitope Affinity Purification | SPECIFICITY This antibody recognizes ~185 kDa of human CD136 protein. It also reacts with mouse and rat.The other species are not tested |
FORMULATION This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer. | STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity.Avoid repeated freezing-thawing cycles. |
Gene ID
4486
Applications/Suggested Working Dilutions
Western Blot 0.1-1 µg/ml | ELISA 0.01-0.1 µg/ml | Immunoprecipitation 2-5µg/ml | IHC 2-10µg/ml | Flow cytometry 5-10µg/ml |
