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苏州工业园区生物纳米园A4#216
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商品描述
Histone H3(Monomethylation at Lys9)
HIST1H3A
Download Product PDFProduct Preparation
Asynthetic peptide containing AR[Me1-K]ST in which Me2-K corresponds to Monomethyl lysine 9 of human histone H3.
Background
Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The methylation of specific residues in the histone tails is a central modification for regulatingepigenetic transitions in chromatin. Whereas methylation of histone H3 on lysine 4, 36, and 79 has been linked with gene activation, methylation of H3 on lysines 9, 27 and histone H4 on lysine 20 is associated with heterochromatin and some repressed genes with euchromatin.Modified lysine residues can exist in a mono-, di-, or tri-methylated state, while the arginine residues can be mono- or di-methylated.Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin.This antibody is specific for histone H3 mono-methylated at K9. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. The antibody will react with any of the above species where the monomethylation modification is present.
PURIFICATION The Rabbit IgG is purified by site-modified Epitope Affinity Purification. | SPECIFICITY This antibody only recognizes ~ 17 kDa of Mono-methylated Histone H3 at K9. It does not cross react to non-methylated H3 or di-, Tri-methylated Histone H3. |
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer. | STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity.Avoid repeated freezing-thawing cycles. |
Gene ID
8350
Applications/Suggested Working Dilutions
Western Blot 0.1-1 µg/ml | ELISA 0.01-0.1 µg/ml | Immunoprecipitation 2-5µg/ml | IHC 2-10µg/ml | Flow cytometry 5-10µg/ml |
