ThismousemonoclonalantibodywasgeneratedagainstplantactinACT11andrecognizessubclasses2and3ofvegetativeandsubclasses4and5ofpollenspecificplantactins(arABIdopsisACT1,3,4,7,11,12).
ActinexistsasaubiquitousproteininvolvedwithfilamentformationthatmakeuplargeportionsoftheCytoskeleton.Actinfilamentsinteractwithmyosintoassistinmusclecontractionaswellasaidingincellmotilityandcytokinesis.Invertebratestherearethreegroupsofactinisoforms:alpha,betaandgamma.Thealphaactinsarefoundinmuscletissuesandareamajorconstituentofthecontractileapparatus.Thebetaandgammaactinsco-existsinmostcelltypesascomponentsofthecytoskeletonandasmediatorsofinternalcellmotility.
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
PartofTheInvestigator"sAnnexeprogram.
| ProductType: | Antibody |
| AccessionID: | AEE75151 |
| Antigen: | ArabidopsisActin(ACT11) |
| MolecularWeight: | 45kDa |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| CloneName: | mAb2345a(2G-10) |
| Reactivity: | Plantactins,othersnottested |
| Immunogen: | PurifiedArabidopsisactin(ACT11) |
| SpeciesImmunized: | Mouse |
| Epitope: | Thr43inplantactin,Thr41inDictyostelium |
| PurificationMethod: | ProteinGcolumn |
| Buffer: | 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide |
| TestedApplications: | WB,IF |
| Storage: | -20C |
| Shipped: | Coldpacks |
| Antibody | Reactivity |
| Actin[mAbGEa] | Universal |
| Actin-1[mAb45a] | ACT1,3,4,12 |
| Actin-11[mAb2345a] | ACT1,3,4,7,11,12 |
| Actin-2[mAb13a] | ACT2,8,11 |
| Actin-8[mAbGPa] | ACT1,2,3,4,7,8,11,12 |
WesternBlotAnalysis
ImmunofluorescenceStainingAnalysis
Adaptedfrom:Kandasamy,M.K.,etal.(2001)PlantCell13,1541-54.
- Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
- Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
- Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
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