GradeUltra Pure Grade

DescriptionDNase I is a recombinant form of DNase I which is RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.DNase I manufactured using state-of-the-art processes yielding animal-free material. The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity of RT-PCR. It is an important tool for all applications requiring DNA-free RNA templates and achieving reliable results with undegraded and stable RNA.

Specification

Appearance	Colorless to light yellow, clear solution.
Source	Bovine pancreas.
Unit Definition	One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.

StorageStore at -20°C

 

Assay	Resuls
Volume act. with calf thymys DNA (Kunitz)	11875 u/ml
Volume act. with calf thymys DNA (Kunitz)	993 KU/ml
Protease up to 50U w.casein resorufinm	Not detectable.
RNases up to 10U with MS II RNA	Not detectable.

Ordering Information

Catalog No	Description	Pack Size
PC0719 - 50kU	Deoxyribonuclease I (DNase I) - For RNA work	50kU
PC0719 - 100kU	Deoxyribonuclease I (DNase I) - For RNA work	100kU

DownloadMSDS

Deoxyribonuclease I (DNase I) - For RNA work

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。