Concentration5-20u/μl5"…CCG↓CTC…3" 3"…GGC↑GAG…5"

Reaction Conditions 1X Buffer V5 30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation65°C for 20 minutes

Ligation / Recutting Assay After 5-fold overdigestion with AccBSI, 90% of the DNA fragments can be ligated and of these 50% can be recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccBSI for 16 hours at 37°C.Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Catalog No	Description	Pack Size
RE1110	AccBSI {BsrBI}	500u

 

DownloadsManualAccBSI {BsrBI}

VivanTechnologies的用作琼脂糖和聚丙烯酰胺凝胶电泳的分子量标准。适用于确定PCR产物或其他双链DNA片段的大小。与其他带相比，大小为250bp的片段强度更高，可以用作定向点。