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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Antigen Information
Gene Symbols:
CCL2 | CCL3 | CCL4 | CCL5 | CSF2 | CXCL1 | CXCL2 | CXCL3 | CXCL8 | IFNG| IL10 | IL12A | IL13 | IL1A | IL1B | IL2 | IL4 | IL5 | IL6 | MMP9 | TNF | VEGFA View more Hide
Assay Format
Number of Targets Detected:
20
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| GRO alpha/beta/gamma
| IFN-gamma
| IL-1 alpha(IL-1 F1)
| IL-1 beta(IL-1 F2)
|
IL-10
| IL-12 p70
| IL-13
| IL-2
| IL-4
|
IL-5
| IL-6
| IL-8(CXCL8)
| MCP-1(CCL2)
| MIP-1 alpha(CCL3)
|
MIP-1 beta(CCL4)
| MMP-9
| RANTES(CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Chen T., Li H., Lin J., et al. Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array. Tuberculosis (Edinb). 2016 Mar;97:108-17. doi: 10.1016/j.tube.2015.12.007. Species: HumanSample type: Serum
- Yang J., Fang P., Yu D., et al. Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation. Circ Res. 2016 Nov 11;119(11):1226-1241.Species: HumanSample type: Serum
- Chen S., Zha X., Shi L., et al. Upregulated TCR? improves cytokine secretion in T cells from patients with AML. J Hematol Oncol. 2015; 8: 72. Published online 2015 Jun 18. doi: 10.1186/s13045-015-0170-0Species: HumanSample type: Conditioned Media
- Zhan Y., Zou S., Hua F., Li F., Ji L., Wang W., Ye Y., Sun L., Chen H., Cheng Y. High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia. Immunology Letters Volume 160, Issue 1, July 2014, Pages 33-38Species: HumanSample type: Serum
- Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354Species: HumanSample type: Plasma
- Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.Species: HumanSample type: Serum
- KrÃ?nkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease."?Circulation?(2012): CIRCULATIONAHA-112.Species: HumanSample type: Conditioned Media
- LÃ?nne, M., Lavrentieva, A., Walter, J. G., & Kasper, C. (2013). Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord.?Cell and tissue research,?353(1), 117-122.Species: HumanSample type: Cell Lysate
- Logan, Patrick, Julia Burnier, and Miguel N. Burnier Jr. "Vascular endothelial growth factor expression and inhibition in uveal melanoma cell lines."?ecancermedicalscience?7 (2013).Species: HumanSample type: Conditioned Media
- Varnum S., Springer D., Chaffee M., et al. The effects of low-dose irradiation on inflammatory response proteins in a 3D reconstituted human skin tissue model. Radiat Res. 2012 Dec;178(6):591-9. doi: 10.1667/RR2976.1.Species: HumanSample type: Tissue Lysate
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