品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
- EGFR
- ERBB2
- ERBB3
- ERBB4
Assay Format
Pathway:
- HER/ErbB Signaling
Number of Targets Detected:
17
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
EGFR(P-Tyr845)
| EGFR(P-Tyr992)
| EGFR(P-Tyr1045)
| EGFR(P-Tyr1068)
| EGFR(P-Tyr1086)
|
EGFR(P-Tyr1148)
| EGFR(P-Tyr1173)
| EGFR(P-Ser1046/1047)
| EGFR(P-Ser1070)
| ErbB2(P-Tyr877)
|
ErbB2(P-Tyr1112)
| ErbB2(P-Tyr 1221/1222)
| ErbB2(P-Tyr1248)
| ErbB2(P-Thr686)
| ErbB2(P-Ser1113)
|
ErbB3(P-Tyr1289)
| ErbB4(P-Tyr1284)
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human EGFR Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set I
- Phosphatase Inhibitor Cocktail II
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792. Species: HumanSample type: Cell Lysate
- Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430Species: HumanSample type: Cell Lysate
- Tang Q., Wang Y., Ma L., et al. Peiminine serves as an adriamycin chemosensitizer in gastric cancer by modulating the EGFR/FAK pathway. Oncol Rep. 2018 Mar;39(3):1299-1305. doi: 10.3892/or.2018.6184.Species: HumanSample type: Cell Lysate
- Asif M., Shafaei A., Abdul Majid A., et al. Mesua ferrea stem bark extract induces apoptosis and inhibits metastasis in human colorectal carcinoma HCT 116 cells, through modulation of multiple cell signalling pathways. Chin J Nat Med. 2017 Jul;15(7):505-514. doi: 10.1016/S1875-5364(17)30076-6.Species: HumanSample type: Cell Lysate
- Kato K., Iwama H., Yamashita T., et al. The anti-diabetic drug metformin inhibits pancreatic cancer cell proliferation in vitro and in vivo: Study of the microRNAs associated with the antitumor effect of metformin. Oncol Rep. 2016 Mar;35(3):1582-92. doi: 10.3892/or.2015.4496Species: HumanSample type: Tissue Lysate
- Mohan N., Shen Y., Endo Y., et al. Trastuzumab, but Not Pertuzumab, Dysregulates HER2 Signaling to Mediate Inhibition of Autophagy and Increase in Reactive Oxygen Species Production in Human Cardiomyocytes. Mol Cancer Ther. 2016 Jun;15(6):1321-31. doi: 10.1158/1535-7163.MCT-15-0741.Species: HumanSample type: Cell Lysate
- Rios-Doria J., Sabol D., Chesebrough J., et al. A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways. Mol Cancer Ther. 2015 Jul;14(7):1637-49. doi: 10.1158/1535-7163.MCT-14-1040. Species: HumanSample type: Tissue Lysate
- Montermini L., Meehan B., Farnier D., et al. Inhibition of oncogenic epidermal growth factor receptor kinase triggers release of exosome-like extracellular vesicles and impacts their phosphoprotein and DNA content. J Biol Chem. 2015 Oct 2;290(40):24534-46. doi: 10.1074/jbc.M115.679217. Species: HumanSample type: Extracellular Vesicles
- Arnold C., et al. Rac1 as a multifunctional therapeutic target to prevent and combat cancer metastasis. Oncoscience 2014, Vol.1, No.8Species: HumanSample type: Cell Lysate
- Panupinthu N., et al. Self-reinforcing loop of amphiregulin and Y-box binding protein-1 contributes to poor outcomes in ovarian cancer. Oncogene (2014) 33, 2846-2856; doi:10.1038/onc.2013.259Species: HumanSample type: Cell Lysate
Prev
123
Next04/24/2018, 08:38 PM
Manual
SDS
Analysis Tool
