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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
- CSF2
- CXCL8
- IFNG
- IL10
- IL12A
- IL1B
- IL4
- IL6
- TGFB1
- TNF
Assay Format
Pathway:
- JAK/STAT Signaling
- MAPK Signaling
- NFkB Signaling
Number of Targets Detected:
10
Species Detected:
- Porcine (pig)
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta(IL-1 F2)
| IL-10
| IL-12 p70
|
IL-4
| IL-6
| IL-8(CXCL8)
| TGF beta 1
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Porcine Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.Species: PorcineSample type: Vitreous Fluid
- Goetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022Species: PorcineSample type: Synovial Fluid
- Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048Species: PorcineSample type: Serum
- Beltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14Species: PorcineSample type: Serum
- Butz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340BSpecies: PorcineSample type: Plasma
- Pazos, M. R., Mohammed, N., Lafuente, H., Santos, M., Martínez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic?ischemic newborn pigs: Role of 5HT 1A and CB2 receptors.?Neuropharmacology,?71, 282-291.Species: PorcineSample type: Tissue Lysate
- Mulloy DP, Stone ML, Crosby IK, et al.?Ex Vivo?Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function.?The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.Species: PorcineSample type: BAL Fluid
University of Memphis11/15/2019, 05:25 PM
Manual
SDS
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