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RayBiotech/Human Apoptosis Signaling Array C1/2 Sample Kit/AAH-APOSIG-1-2

价格
面议
货号:AAH-APOSIG-1-2
浏览量:127
品牌:RayBiotech
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商品描述

Antigen Information

Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CHEK1 | CHEK2 | EIF2A | HSPB1 | MAP3K7 View more Hide

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • mTOR Signaling
  • NFkB Signaling
  • p53 Signaling
  • PI3K-AKT Signaling
  • TGF-beta Signaling
  • Wnt/beta-Catenin Signaling
Number of Targets Detected:
19
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)(PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA
ATM (P-Ser1981)
  • Gene Symbol: ATM
  • Gene ID: 472
  • Accession #: Q13315
  • ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA
BAD (P-Ser112)
  • Gene Symbol: BAD BBC6 BCL2L8
  • Gene ID: 572
  • Accession #: Q92934
  • ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA
Caspase-3 (Cleaved Asp175)
  • Gene Symbol: CASP3 CPP32
  • Gene ID: 836
  • Accession #: P42574
  • ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA
Caspase-7 (Cleaved Asp198)
  • Gene Symbol: CASP7 MCH3
  • Gene ID: 840
  • Accession #: P55210
CHK1 (P-Ser296)
  • Gene Symbol: CHEK1 CHK1
  • Gene ID: 1111
  • Accession #: O14757
CHK2 (P-Thr68)
  • Gene Symbol: CHEK2 CDS1 CHK2 RAD53
  • Gene ID: 11200
  • Accession #: O96017
  • ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA
eIF-2a (P-Ser52)
  • Gene Symbol: EIF2A CDA02 MSTP004 MSTP089
  • Gene ID: 83939
  • Accession #: Q9BY44
  • ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5595/5594
  • Accession #: P27361/P28482
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA
HSP27 (P-Ser82)
  • Gene Symbol: HSPB1 HSP27 HSP28
  • Gene ID: 3315
  • Accession #: P04792
  • ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32)
  • Gene Symbol: NFKBIA IKBA MAD3 NFKBI
  • Gene ID: 4792
  • Accession #: P25963
  • ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA
JNK (P-Thr183/Tyr185)
  • Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C
  • Gene ID: 5599
  • Accession #: P45983
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA
NFKBP65 (P-Ser536)
  • Gene Symbol: RELA NFKB3
  • Gene ID: 5970
  • Accession #: Q04206
  • ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA
PARP1 (Cleaved Asp214/Gly215)
  • Gene Symbol: PARP1 ADPRT PPOL
  • Gene ID: 142
  • Accession #: P09874
  • ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA
p27 (P-Thr198)
  • Gene Symbol: PSMD9
  • Gene ID: 5715
  • Accession #: O00233
  • ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
P53 (P-Ser15)
  • Gene Symbol: TP53 P53
  • Gene ID: 6198
  • Accession #: P23443
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA
SMAD2 (Ser245/250/255)
  • Gene Symbol: SMAD2 MADH2 MADR2
  • Gene ID: 4087
  • Accession #: Q15796
  • ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885
  • Accession #: O43318
  • ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Signaling Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP"s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Bao X, Shi J, Xie F, et al. Proteolytic Release of the p75NTR?Intracellular Domain by ADAM10 Promotes Metastasis and Resistance to Anoikis.?Cancer Res. 2018;78(9):2262-2276. doi:10.1158/0008-5472.CAN-17-2789Species: Human Sample type: Cell Lysates
  2. Smolensky D, Rhodes D, McVey DS, et al. High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis.?J Med Food. 2018;21(10):990-998. doi:10.1089/jmf.2018.0008Species: HumanSample type: Cell Lysates
The human apoptosis array provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.
Moffitt Cancer Center
This apoptosis kit was very user-friendly and gave detailed instructions on how to analyze findings using a software like Excel. This assay was very helpful for examining multiple gene targets simultaneously, so we got a lot of data out of 1 experiment. Negative and positive controls were included on the blots, which was useful in subsequent analyses.Notes: the Caspase3 antibody seemed a lot more sensitive than other antibodies on the blot. Also, some of the gene names in the kit manual differed from those listed on the website, so keep that in mind when analyzing data to ensure that the correct gene is being referred to.
Medical College of Wisconsin
It seems antibodies are not evenly attached on the membrane. Some signals show up outside of spots. It has high background when I follow up the suggested procedures. I cannot see any benefit of this array compared to other array such as apoptosis array or other company's similar product.
Penn State College of Medicine
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