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蚂蚁淘在线 / 品牌中心 / RayBiotech / RayBiotech/Lung Cancer IgG Autoantibody Array G1/8 Sample Kit/PAH-LCAG-G1-8
商品描述
Assay Format
Number of Targets Detected:
30
Species Detected:
- Human
Compatible Sample Types:
- Hydrothorax
- Plasma
- Serum
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
8 or 16 Sample Kit
Product Feature
- Small sample volume required (2 µl)
- Detects antibody specificity
- Detects autoantibody expression
- Affordable, quick and simple to use
Target Name
Scroll over each spotted protein for more information | ||||
14-3-3 theta
| Annexin A1
| Annexin A2
| CAGE
| Cathepsin D
|
CDK2
| c-Myc(MYC)
| Cyclin A
| Cyclin B1
| Cyclin D1
|
DKK-1
| GBU4-5
| HSP70-9B/HSPA9/GRP-75
| Hu-D
| IMDH2
|
IMP1
| IMP2/IGF2BP2/p62
| IMP3
| LAMR1
| MAGEA4
|
MALAT1
| NY-ESO-1
| p53
| Paxillin
| PGAM1
|
PGP9.5
| Recoverin
| SOX2
| Survivin(BIRC5)
| Ubiquilin-1
|
Application Notes
Suggested Application
Lung cancer IgG autoantibody profiling
Kit Components
- RayBio® Human Lung Cancer IgG Autoantibody G1 Array Slide
- RayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
- Blocking Buffer (8 ml/bottle)
- Wash Buffer 1 (30 ml/bottle)
- Wash Buffer 2 (30 ml/bottle)
- 1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
- 1,500X Streptavidin-Conjugated Fluor (1 µl/vial)
Other Materials Required
- Small plastic boxes or containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- Distilled water
- Aluminum foil
- Laser scanner for fluorescence detection
Protocol Outline
- See Manual for relevant protocols.
Typical Data
The following image is a typical result of the Human Lung Cancer Autoantibody Protein Array. The arrays were incubated with 1:200 diluted serum samples. The results shown in Fig. A indicate no background from the biotin-labeled anti-human IgG antibody or from the fluorescent dye-labeled streptavidin. In Fig. B, a base background level is established from normal serum. This background can be subtracted during data analysis without affecting the results. Lastly, Figures C and D are the results of two individuals diagnosed with lung cancer. Several autoantibodies show clear binding.
Fig 1. Human serum test of RayBio® Lung Cancer Autoantibody Detection Array. The protein arrays were respectively incubated with either no serum (A) or with diluted serum samples derived from either normal human (B), lung cancer patient A (C), or lung cancer patient B (D). Biotin labeled BSA positive controls are outlined in red. Human IgG positive controls are outlined in white.
Storage/Stability
Upon receipt, all components of the RayBio® Human Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at –20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.
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Manual
SDS
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