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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Antigen Information
Gene Symbols:
CCL20 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B | IL2| IL21 | IL22 | IL23A | IL4 | IL5 | IL6 | TGFB1 | TNF View more Hide
Assay Format
Number of Targets Detected:
18
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 beta(IL-1 F2)
| IL-10
| IL-12 p70
| IL-13
|
IL-17A
| IL-17F
| IL-2
| IL-21
| IL-22
|
IL-23 p19
| IL-28A(IFN-lambda 2)
| IL-4
| IL-5
| IL-6
|
MIP-3 alpha(CCL20)
| TGF beta 1
| TNF alpha
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Th1/Th2/Th17 Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Velsko I., Chukkapalli S., Rivera-Kweh M., et al. Periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in ?v?6 integrin-deficient mice. Infect Immun. 2015 Dec;83(12):4582-93. doi: 10.1128/IAI.01077-15.Species: HumanSample type: Conditioned Media
- Che N. et al. Impaired B cell Inhibition by Lupus Bone Marrow Mesenchymal Stem Cells is Caused by Reduced CCL2 Expression. J Immunol 2014; 193:5306-5314; Prepublished online 22. doi: 10.4049/jimmunol.1400036Species: MouseSample type: Conditioned media
- Hou T., Li Z., Luo F., Xie Z., Wu X., Xing J., Dong S., Xu J. A composite demineralized bone matrix e Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow. Biomaterials, Available online 19 April 2014. [Epub ahead of print]Species: MouseSample type: Tissue Lysate
- Hou, Ni, et al. "A novel chronic stress-induced shift in the Th1 to Th2 response promotes colon cancer growth."?Biochemical and biophysical research communications?439.4 (2013): 471-476.Species: MouseSample type: Serum
- Marchesan, Julie Teresa, et al. "Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis."?Arthritis research & therapy?15.6 (2013): R186.Species: MouseSample type: Serum
- Abdi K., Sing N., Matzinger P. Lipopolysaccharide-activated dendritic cells: "exhausted" or alert and waiting? J Immunol. 2012 Jun 15;188(12):5981-9. doi: 10.4049/jimmunol.1102868. Species: MouseSample type: Conditioned Media
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